色谱检测技术同其他方法比较优势
通过色谱检测技术,BACT/ALERT® 培养瓶提供一种同其他方法比较更为精良的检测技术。在所有的培养瓶底部固化有特制的液体乳剂传感器(LES),可以受微生物代谢产生的 CO2 导致 pH 值变化导致颜色改变。BacT/ALERT® 3D 全自动细菌检测系统每 10 分钟检测并分析瓶底颜色的变化。通过复杂的算法(包括特有的“阈值”法),来确保尽早的检出微生物。当发生延迟放瓶时,在放入仪器前即可通过肉眼检测瓶底颜色来报告阳性瓶。这样可以保证准确和快速的复苏不同标本中存在的各种微生物。

- 色谱技术和复杂的算法将因延时放瓶导致的漏检降至最低
- 低假阳性率
- 直观,不可逆的颜色变化
灵活的培养瓶提供可靠的复苏率
BacT/ALERT® 提供各种培养瓶全方位保证各种各样的微生物包括细菌,分枝杆菌,真菌和酵母菌的快速恢复。对于各种类型的标本包括血液,无菌体液和痰,BacT/ALERT® 培养瓶可以在 72 小时以内检测出98%的微生物。*
- FDA 批准可以用于血液,无菌体液和血小板
- 可以复苏各种各样的微生物
- 专用的复苏分枝杆菌的培养瓶
- 可中和抗生素的培养瓶可以优化经过抗菌药物治疗的患者的培养瓶阳性率
*Bourbeau PP and Pohlan JK. Three days of incubation may be sufficient for routine blood cultures withBACT/ALERT® FAN® blood culture bottles. J Clin Microbiol. 2001; 39(6): 2079-82.
新的 FAN® Plus 培养瓶:提升检出时间和阳性率
我们下一代的聚合物吸附珠中和抗生素培养瓶(FAN® Plus)可以提升检出时间和阳性率。FAN® Plus培养瓶易于进行革兰染色观察,聚合物吸附珠(APB)提供抗生素吸附
- 提升阳性率
- 新的改良的培养基配方提供更适于细菌和酵母菌的生长环境
- 可以适用于血液和无菌体液中各种微生物的生长
- 提升延迟放瓶条件下的生长表现
- 提升检出时间
- 改进的中和抗生素能力,提供很好的细菌生长和检出条件
安全有效 :塑料培养瓶的引领者
生物梅里埃率先推出了革命性的多重的塑料材质的培养瓶。具备独特的聚碳酸酯材料,三层设计保证了各种微生物的分离率。防碎培养瓶通过消除以外泄露,潜在生物危害和玻璃割伤来提升实验室安全。因为培养瓶的重量较轻,这些培养瓶可以降低运输和处理成本。多种研究表明:在保证这些优点的同时,我们的培养瓶不会影响到检出率和检出时间。
- 防碎设计避免潜在的生物危害
- 同玻璃比较重量减少2/3
- 减少储存,运输和处理成本
- 多重、不透气的设计来保证培养瓶的需氧和厌氧环境
- 适用于各种气动传输系统,无需特殊容器

标准培养基
|
血培养瓶类型 |
培养基组成 |
标本类型 |
标本量 |
 |
BACT/ALERT® SA 标准需氧瓶
- Ref. 259789
|
40 ml补充胰蛋白酶大豆肉汤 (TSB)
|
血液或无菌体液(SBF)
|
大于10 ml |
 |
BACT/ALERT® SN 标准厌氧瓶
- Ref. 259790
|
40 ml 补充胰蛋白酶大豆肉汤 TSB |
血液或无菌体液(SBF) |
大于10 ml |
苛养菌抗菌剂中和 Plus 培养基 (FAN® Plus)
|
培养瓶类型 |
培养基组成 |
标本类型 |
标本量 |
 |
BACT/ALERT® FA Plus - 货号 410851
|
30 ml 复合培养基加吸附树脂
|
血液或无菌体液(SBF)
|
大于10 ml |
 |
BACT/ALERT® FN Plus - 货号 410852
|
40 ml 复合培养基加吸附树脂
|
血液或无菌体液(SBF) |
大于10 ml |
 |
BACT/ALERT® PF Plus - 货号 410853
|
30 ml 复合培养基加吸附树脂
|
血液 |
大于 4 ml |
苛养菌抗菌剂中和培养基
含可中和抗菌剂的活性炭的 FAN® 培养基
|
培养瓶类型 |
培养基组成 |
标本类型 |
标本量 |
 |
BACT/ALERT® FA FAN® Aerobic - 货号 259791 |
30 ml 的含脑心浸出液的浓缩TSB,加活性炭 |
血液或无菌体液(SBF) |
大于 10 ml |
 |
BACT/ALERT® FN FAN® Anaerobic - 货号 259793 |
40 ml 的含脑心浸出液的浓缩TSB,加活性炭 |
血液或无菌体液(SBF) |
大于10 ml |
 |
BACT/ALERT® PF Pediatric FAN® - 货号 259794 |
20 ml 的含脑心浸出液的浓缩TSB,加活性炭 |
血液 |
大于 4 ml |
分枝杆菌培养基
|
培养瓶类型 |
培养基配方 |
样本类型 |
样本量 |
 |
BACT/ALERT® MP 分枝杆菌检测流程 - 配合使用货号 259797 的产品与货号 259760 的产品 |
10 ml 7H9 Middlebrook 肉汤 |
痰标本或血液以外的无菌体液 |
0.5 ml |
血小板 QC 检测
|
培养瓶类型 |
培养基配方 |
样本类型 |
样本量 |
 |
BACT/ALERT® BPA 血小板好氧菌培养瓶– 货号 279018 或 EU 货号 279044 |
40 ml 胰蛋白胨大豆肉汤 (TSB) 补充培养基 |
去白细胞的血浆分离血小板 (LRAP) 单元,单个或多达六 (6) 单位白细胞减除的全血浓缩血小板 (LRWBPC) 并袋 |
4 – 10 ml |
 |
BACT/ALERT® BPN BPN 血小板厌氧菌培养瓶 - 货号. 279019 或 EU 货号 279045 |
40 ml 胰蛋白胨大豆肉汤 (TSB) 补充培养基 |
去白细胞的血浆分离血小板 (LRAP) 单元,单个或多达六 (6) 单位白细胞减除的全血浓缩血小板 (LRWBPC) 并袋 |
4 – 10 ml |
BACT/ALERT® 持针器
|
说明 |
产品号 |
包装 |
 |
BACT/ALERT® 帽形持针器 适用于传统的安全血培养采血 |
279012 |
120 |
 |
BACT/ALERT® 内嵌型持针器 可以连接真空采血管,方便采血后收集其他标本。 |
279013 |
60 |
Greiner Bio-One VACUETTE® 安全釆血套件和大容量接头*
|
说明 |
产品号 |
包装 |
 |
VACUETTE® 安全采血套件 无菌包装,含针头、采血管和 Greiner 接头。 |
货号 413251 - 针号 21G,30cm 采血管。货号 413201 - 针号 23G,30cm 采血管。货号 415419 - 针号 21G,19cm 采血管。货号 415450 - 针号 23G,19cm 采血管。 |
每箱 240 个 |
 |
大容量无菌接头
无菌接头封盖采用改良的颈部设计,可适配真空采血管,无需使用其他插件。(母鲁尔接头)。
|
413200 |
320 |
- 实际操作培训课程
- 本地电话技术支持
- 其他按市场提供的服务和支持
建议采血程序

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BACT/ALERT® 3D: 文献
标准再分离培养基:
Mirret S, Reller LB, Petti CA, Woods CW, Vazirani B, Sivadas R, and Weinstein MP. Controlled Clinical Comparison of BACT/ALERT® Standard Aerobic Medium with BACTEC Standard Aerobic Medium for Culturing Blood. J. Clin Microbiol. 2003; 41(6):2391-2394.
Bernard D, Verschraegen G, Claeys G, Lauwers S, Rosseel P. Bilophila wadsworthia BACTeremia in a patient with gangrenous appendicitis. Clin Infect Dis. June 1994;18:1023-1024.
标准与 FAN® 培养基:
Weinstein MP, Mirrett S, Reimer LG, Wilson ML, Smith-Elekes S, Chuard CR, Joho KL, Reller LB. Controlled evaluation of BACT/ALERT standard aerobic and FAN® aerobic blood culture bottles for detection of BACTeremia and fungemia. J Clin Microbiol. 1995;33(4):978-981.
Wilson ML, Weinstein MP, Mirrett S, Reimer LG, Feldman RJ, Chuard CR, Reller LB. Controlled evaluation of BACT/ALERT® standard anaerobic and FAN® anaerobic blood culture bottles for the detection of BACTeremia and fungemia. J Clin Microbiol. 1995;33(9):2265-2270.
McDonald LC, Fune J, Gaido LB, Weinstein MP, Reimer LG, Flynn TM, Wilson ML, Mirrett S, Reller LB. Clinical importance of increased sensitivity of BACT/ALERT® FAN® aerobic and anaerobic blood culture bottles. J Clin Microbiol. 1996;34(9):2180-2184.
Mirrett S, Everts RJ, Reller LB. Controlled Comparison of Original Vented Aerobic FAN® Medium with New Nonvented BACT/ALERT® FA Medium for Culturing Blood. J Clin Microbiol. 2001;39(6):2098-2101.
Mirrett S, Petti CA, Woods CW, Magadia R, Weinstein MP, Reller LB. Controlled Clinical Comparison of the BACT/ALERT® FN and the Standard Anaerobic SN Blood Culture Medium. J Clin Microbiol. 2004;42(10):4581-4585.
FAN® 与 BACTEC Plus:
Ziegler R, Johnscher I, Martus P, Lenhardt D, Just HM. Controlled clinical laboratory comparison of two supplemented aerobic and anaerobic media in automated blood culture systems to detect bloodstream infections. J Clin Microbiol. 1998; 36(3):657-661.
Pohlman JK, Kirkley BA, Easley KA, Basille BA, Washington JA. Controlled clinical evaluation of BACTEC Plus Aerobic/F and BACT/ALERT® aerobic FAN® bottles for detection of bloodstream infections. J Clin Microbiol. 1995;33(11):2856-2858.
Jorgensen JH, Mirrett S, McDonald LC, Murray PR, Weinstein MP, Fune J, Trippy CW, Masterson M, Reller LB. Controlled clinical laboratory comparison of BACTEC Plus Aerobic/F Resin medium with BACT/ALERT® Aerobic FAN® medium for detection of BACTeremia and fungemia. J Clin Microbiol. 1997;35(1):53-58.
FAN Plus 培养基:
Altun O, Almuhayawi M, Ullberg M and Ozenci V, Clinical Evaluation of the FilmArray Blood Culture Identification Panel in Identification of BACTeria and Yeasts from Positive Blood Culture Bottles, J. Clin. Microbiol. 2013, 51(12):4130.
Kirn TJ, Mirrett S, Reller LB, Weinstein MP, Controlled Clinical Comparison of BACT/ALERT FA Plus and FN Plus Blood Culture Media with BACT/ALERT FA and FN Blood Culture Media, J. Clin. Microbiol. doi:10.1128/JCM.03063-13.
Lee DH, Kim SC, Bae IG, Kim S, Clinical Evaluation of BACT/Alert FA Plus and FN Plus Bottles Compared with Standard Bottles, J. Clin. Microbiol. 2013, 51(12):4150.
Mitteregger D, Barousch W, Nehr M, Kundi M, Zeitlinger M , Makristathis A and Hirschl AM, Neutralization of Antimicrobial Substances in New BACT/Alert FA and FN Plus Blood Culture Bottles, J. Clin. Microbiol. 2013, 51(5):1534.
体液培养:
Bourbeau P, Riley J, Heiter BJ, Master R, Young C, Pierson C. Use of the BACT/ALERT® blood culture system for culture of sterile body fluids other than blood. J Clin Microbiol. 1998;36(11):3273-3277.
Alfa MJ, Degagne P, Olson N, Harding KM. Improved detection of BACTerial growth in continuous ambulatory peritoneal dialysis effluent by use of BACT/ALERT® FAN® bottles. J Clin Microbiol. 1997;35(4):862-866.
玻璃瓶与塑料瓶:
Snyder JW, Munier GK, Bostic GD, Bozigar PS, Hanna R. Evaluation of a Plastic Nonvented Aerobic Blood Culture bottle for Use with the BACT/ALERT® Microbial Detection System. J Clin Microbiol 2002; 40(12): 4757-4759.
Petti, CA, Mirrett S, Woods CW, and Reller LB. Controlled Clinical Comparison of Plastic versus Glass Bottles of BACT/ALERT® PF Medium for Culturing Blood from Children. J Clin Microbiol 2005; 43(1): 445–447
Petti CA, Mirrett S, Woods CW, Reller LB, Controlled clinical comparison of plastic and glass bottles of BACT/ALERT® FA medium for culturing organisms from blood of adult patients. J Clin Microbiol 2005; 43(4), 1960-1962
儿科:
Alpern E, Alessandrini E, Bell L, Shaw K, McGowan L. Occult BACTeremia from a pediatric emergency department: current prevalence, time to detection, and outcome. Pediatrics. 2000;106(3):505-511.
McGowan K, Foster J, Coffin S. Outpatient pediatric blood cultures: time to positivity. Pediatrics. 2000;106(2):251-255.
Krishner KK, Gibb P, CorbettS, and Church DEIDRE. Comparison of the BACT/ALERT® PF Pediatric FAN® Blood Culture Bottle with the Standard Pediatric Blood Culture Bottle, the Pedi-BACT J Clin Microbiol. 2001;39(8): 2880-2883.
BACT/ALERT® 与 ESP:
Welby-Sellenriek PL, Keller DS, Ferrett RJ, Storch GA. Comparison of the BACT/ALERT® FAN® aerobic and the Difco ESP 80A aerobic bottles for pediatric blood cultures. J Clin Microbiol. 1997;35(5):1166-1171.
Doern G, Barton A, Rao S. Controlled comparative evaluation of BACT/ALERT® FAN® and ESP 80A aerobic media as means for detecting BACTeremia and fungemia. J Clin Microbiol. 1998; 35(9):2686-2689.
分枝杆菌、酵母菌和真菌:
Alcaide F, Benitez MA, Escriba JM, Martin R. Evaluation of the BACTEC MGIT™ 960 and the MB/BACT systems for recovery of mycoBACTeria from clinical specimens and for species identification by DNA AccuProbe. J Clin Microbiol. 2000;38(1):398-401.
Crump J, Tanner DC, Mirrett S, McKnight CM, Reller LB, Controlled Comparison of BACTEC 13A, Myco/F Lytic, BACT/ALERT® MB and Isolator 10 Systems for Detection of MycoBACTeremia. J Clin Microbiol 2003;41(5):1987-1990.
McDonald, L., Weinstein, M., Fune, J., Mirrett, S., Reimer, L., Reller, B., Controlled Comparison of BACT/ALERT® FAN® Aerobic Medium and BACTec Fungal Blood Culture Medium for Detection of Fungemia, J Clin Microbiol 2001:39(2): 622–624.
其他:
Paul P. Bourbeau and Janice K. Pohlman, Three Days of Incubation May Be Sufficient for Routine Blood Cultures with BACT/ALERT® FAN® Blood Culture Bottles. J Clin Microbiol. 2001;39(6):2079-2082.
Seegmuller I, Eschenbach U, Kamereck K. Sensitivity of the BACT/ALERT® FA medium for detection of Pseudomonas aeruginosa in pre-incubated blood cultures and its temperature dependence. J Med Microbiol, (2004); 53. 869-874.